Methodology
Essay by Aiman Amreel • August 4, 2015 • Study Guide • 502 Words (3 Pages) • 824 Views
Methodology
- Chemicals
There are some chemicals that have to be used during experimental work during the research
palm oil empty fruit bunch convert to sugar. The list of chemicals as follows:-
Table 1.1 The list of chemicals used
1 | Diluted sulphuric acid |
2 | Deionised water |
3 | 1% of dinitrosalicylic acid |
4 | Stock solution of anhydrous D-gulose at 10 g/L |
5 | Phenol |
6 | Sodium Sulphite |
7 | NaoH |
8 | Potassium sodium tartarate |
- Apparatus
There are some apparatus that have to used during the experimental work in this research
palm oil empty fruit bunch convert to sugar. The list of the apparatus are as follow :
Table 1.2 The list of apparatus used
1 | Micropippete |
2 | U/Visible spectrophotometr |
3 | Autoclaved |
4 | Centrifuge |
5 | Waterbath |
6 | Centrifuge tubes |
- Media preparation
The EFB was collected at a nearby oil palm company, Felda Lepar Hilir 2, Kuantan, Pahang. After
the collection of sample, the EFB was washed with deionized water to remove dirt and debris
That attached it and was cut into smaller pieces. For 3 consecutive days, the EFB was oven-
dried at 80°C . By using a fibre grinder, the dried EFB were grounded to approximately 0.5mm ~
10mm. The particle size was determine by using a sieve tray.
- EFB Pretreatment – Acid Hydrolysis
EFB (10 g ) was added into different concentration of sulphuric acid ( H2SO4) 1%, 2%, 4%, 6%
and autoclaved at 130°C, pressure 10 psi for 1 hour. After reaching the required reaction time,
the reaction mixture was centrifuged for 10 minutes, 12 000 rpm and 10°C. Then, the solid was
separated from the liquid phase by filtration.
1.5. Dinitrosalisalicylic acid (DNS)
The reducing sugar are determined by using dinitrosalicylic Acid (DNS) by standard glucose
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