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Color Reactions of Proteins and Amino Acids

Essay by   •  September 15, 2018  •  Lab Report  •  303 Words (2 Pages)  •  237 Views

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EXPERIMENT NO. 1

PROTEINS

COLOR REACTIONS OF PROTEINS AND AMINO ACIDS


INTRODUCTION

Protein Structures and Importance of its Color Reaction in Amino Acids

        Proteins have complex structures that are described at four levels: primary, secondary, tertiary, and quaternary.

        Protein reacts different reagents to form coloured products. These tests, known as colour reactions of proteins, are significant in qualitative detection and quantitative estimation of proteins, and of their constituent amino acids in body fluids and other biological materials. The written report discusses some of the coloured reactions of proteins based on peptide bonds and type of amino acid residues like Ferric Chloride reaction to determine the presence of phenols, Ninhydrin reaction to detect the presence of amino acid, Xanthoproteic reaction to know the presence of aromatic amino acids, (Hopkins-Cole’s Test) to detect the presence of tryptophan, Sakaguchi Test for Guanidine Group (presence of arginine), test for Lead Acetate for Sulphur-containing Amino Acids (cysteine or cystine)


FERRIC CHLORIDE TEST

Objectives:

In this experiment, we should be able to:

  • To know what is Ferric Chloride Acid
  • To determine the presence of phenols in a given sample.

Procedures:

        

Presentation of the Results

Step 1: Place 5.0 mL of milk on the watch glass and heat over a team bath until a film is produced on the surface of the liquid.

Step 2:

Remove the film by the stirring rod and place it into a clean test tube.

Step 3:

Add 5.0 gtts of FeCl3 solution

Step 4:

Note the change in color of the film

[pic 1]

[pic 2]

[pic 3]

[pic 4]


EXPERIMENTAL RESULTS

SAMPLE

OBSERVATION

BEFORE

AFTER

EVAPORATED MILK MIXED WITH FeCl3

[pic 5]

[pic 6]

The milk hardened or formed a film structure after being heated.

When the film (milk) was mixed with FeCl3 solution, the colour turned out into yellow.

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